国模在线观看_日本va欧美va欧美va精品_久久久久久逼_色综合a_亚洲色偷偷av男人的天堂_蜜桃视频在线入口www_996热re视频精品视频这里_无码纯肉视频在线观看_亚洲精品国产av成拍色拍个_精品久久国产字幕高潮

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠正常細(xì)胞>CRL-2279MS1 小鼠胰島內(nèi)皮細(xì)胞

MS1 小鼠胰島內(nèi)皮細(xì)胞

簡要描述:CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2279
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-09-11
  • 訪  問  量:2678

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞 的詳細(xì)介紹

CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞

ATCC® Number:  CRL-2279™

Designations:  MS1 (MILE SVEN 1)

Depositors:   JL Arbiser

Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]

Shipped:  froz


Organism: Mus musculus (mouse)en

Medium & Serum:  See Propagation

Growth Properties: adherent

Morphology: endothelial


CRL-2279

Source: Organ: pancreas

Strain: C57BL/6

Tissue: islet of Langerhans; endothelium

Cell Type: SV40 transformed

Cellular Products: tissue inhibitor of bioreactive matrix metalloproteinase (high levels)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Receptors: vascular endothelial growth factor (VEGF), expressed

Comments: MS1 is a pancreatic islet endothelial cell line established in 1994.

Primary islet endothelial cells were transduced with a temperature sensitive SV40 large T antigen (tsA-58-3) constructed and screened for resistance to G418.

Resistant colonies were isolated in cloning rings and screened for uptake of diI-Ac-LDL.

The line retains many properties of endothelial cells including uptake of acetylated LDL and expression of both Factor VIII related antigen and VEGF receptor.

The cells are useful for the study of signal transduction of angigenic factors.

It is the only line known that can give rise to benign hemangiomas.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22603: Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347

51452: Arbiser JL, et al. Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR-1 and VEGFR-2 in vivo. Am. J. Pathol. 156: 1469-1476, 2000. PubMed: 10751370


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 兴业县| 宜宾市| 衡阳市| 东至县| 桐庐县| 河源市| 邻水| 江安县| 涿州市| 炉霍县| 黄石市| 德江县| 抚顺县| 木兰县| 思南县| 神农架林区| 曲水县| 左贡县| 桐庐县| 陆丰市| 东城区| 新化县| 札达县| 涿鹿县| 浦江县| 沾化县| 长兴县| 通化县| 离岛区| 开封市| 池州市| 龙门县| 林西县| 林周县| 垣曲县| 南雄市| 民权县| 布拖县| 肇源县| 芦山县| 临海市|